Molecular Cloning and DNA Technology Worksheet

When the recombinant DNA is transformed into the host the host cell is now considered a _______________ ______________.
After ligation of the inserted DNA and the plasmid DNA, you now place the ______________ ________ into a host bacteria cell.
Enzymes called endonucleases or ______________ _______________ "digest" or cut DNA at sequence specific sites.
After digestion of both the DNA you are inserting and the plasmid DNA, the pieces are then ______________ together.
The _________ must be cut so that it is open to accept the bacterial DNA.
The enzyme _______ ___________ is added to a reaction to help bond plasmid DNA and the DNA that is being inserted.
The practice of selectively mating individuals with desired traits. _______________ ____________________
Recombinant plasmid DNA will be taken up into the bacteria cell by a process called ___________________.
_________________ _________________ such as antibiotic resistance genes are added to the recombinant plasmid.
A way to locate a specific gene in one organism, cut the gene out, then transfer the gene into a different organism. _________________ _________________
Genetic structure that will carry the source DNA into the recipient cell. _______________ ____________
Before molecular cloning is complete, the DNA will undergo this type of asexual reproduction. ______________ ____________
What is a GMO? ________________ ________________ ______________
Also known as selective breeding. _______________ _________________